| Gene | Higd1a |
| Chromosome | 9 |
| Deletion Start | 121,852,584 |
| Deletion End | 121,849,622 |
| Deletion Size | 2,963 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 121,852,125 | 121,852,143 | CAGGTGCTTCCCAGAGGAC |
| TUP | 121,852,099 | 121,852,124 | ACTGCATTCTTCCGCCAGTGATGGGC |
| TUR | 121,852,078 | 121,852,096 | TCTGCGTCTGCCAGTGTTG |
| TDF | 121,850,361 | 121,850,381 | CACGTTGTCTTGGCTCCTCTC |
| TDP | 121,850,331 | 121,850,360 | CCGTGCAGCTAACACAGTCACAATTCTTTG |
| TDR | 121,850,305 | 121,850,322 | CTGCAAAGCCCGCCATTC |
PCR Primers
| Primer | Sequence |
| SU | TCACTAACCATAGTAGTTCATTCA |
| SD | AAGCATGGCCTAGTCTGCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 66 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13210A-D5 | VGB6 | C57BL/6NTac | |
| 13210A-E6 | VGB6 | C57BL/6NTac | |
| 13210A-E8 | VGB6 | C57BL/6NTac | |
| 13210A-F6 | VGB6 | C57BL/6NTac | |
| 13210A-F11 | VGB6 | C57BL/6NTac | |
