| Gene | Arl10 |
| Chromosome | 13 |
| Deletion Start | 54,575,098 |
| Deletion End | 54,580,982 |
| Deletion Size | 5,885 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 54,575,438 | 54,575,457 | CCCTGGTAGAGCCCTCATTC |
| TUP | 54,575,458 | 54,575,484 | CCGGTGCCGGATAATCGTGAGATCTTG |
| TUR | 54,575,527 | 54,575,547 | TCGCGCACAATCTTTCTCATC |
| TDF | 54,580,579 | 54,580,600 | AAGGAGGGCACCGAATTTCTTC |
| TDP | 54,580,601 | 54,580,627 | CACAGGATCTAGAGGCACTTCCTGGGA |
| TDR | 54,580,686 | 54,580,706 | AGCCAGCACAGCATGGATGTC |
PCR Primers
| Primer | Sequence |
| SU | AGCCTCACTTCTACCACACTG |
| SD | AGGAACACAAGGCCACAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 128 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13211A-B7 | VGB6 | C57BL/6NTac | |
| 13211A-D5 | VGB6 | C57BL/6NTac | |
| 13211A-F1 | VGB6 | C57BL/6NTac | |
| 13211A-G2 | VGB6 | C57BL/6NTac | |
