| Gene | Gpr84 |
| Chromosome | 15 |
| Deletion Start | 103,309,645 |
| Deletion End | 103,308,441 |
| Deletion Size | 1,205 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 103,309,481 | 103,309,500 | AGCTCCGAACCCGCTTCAAC |
| TUP | 103,309,456 | 103,309,479 | TGCTCATTGCCAACCTCACCCTGG |
| TUR | 103,309,391 | 103,309,412 | CCAATGGAGGTGGAGGTATGTG |
| TDF | 103,308,687 | 103,308,706 | GGGAAGGTGACCCGTATGTG |
| TDP | 103,308,662 | 103,308,686 | CTTCGCAGTGTTCCTCTGCTTCGCC |
| TDR | 103,308,617 | 103,308,637 | CCTGGCGTCCAGAATGTTGAG |
PCR Primers
| Primer | Sequence |
| SU | AAGCTGCCAGGTTTATGGG |
| SD | AAAGCATGCACGCAAGCGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13214A-C5 | VGB6 | C57BL/6NTac | |
| 13214A-A11 | VGB6 | C57BL/6NTac | |
| 13214A-H10 | VGB6 | C57BL/6NTac | |
| 13214A-B12 | VGB6 | C57BL/6NTac | |
| 13214A-G4 | VGB6 | C57BL/6NTac | |
