| Gene | Lyve1 |
| Chromosome | 7 |
| Deletion Start | 110,862,865 |
| Deletion End | 110,852,124 |
| Deletion Size | 10,742 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 110,862,139 | 110,862,159 | GTCCTGGGCAAGTGAAGTAAC |
| TUP | 110,862,102 | 110,862,129 | AAAGAGAACCCTTCTGCTGAAGATGCCC |
| TUR | 110,862,062 | 110,862,084 | TCAACTCCGAACATCTGATCCTG |
| TDF | 110,852,866 | 110,852,886 | TGCTGGCTCTCCTCTTCTTTG |
| TDP | 110,852,842 | 110,852,861 | TGCCGCTGTGCTGGCTGTTT |
| TDR | 110,852,809 | 110,852,828 | AGATGTGCCTGCAACCTCAC |
PCR Primers
| Primer | Sequence |
| SU | CAGTCTCTTAGCGGCAGAGC |
| SD | CTCAAGTCACCCAGGCTCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13216A-A3 | VGB6 | C57BL/6NTac | |
| 13216A-B8 | VGB6 | C57BL/6NTac | |
| 13216A-F5 | VGB6 | C57BL/6NTac | |
| 13216A-F11 | VGB6 | C57BL/6NTac | |
| 13216A-G10 | VGB6 | C57BL/6NTac | |
