| Gene | BC004004 |
| Chromosome | 17 |
| Deletion Start | 29,282,082 |
| Deletion End | 29,301,538 |
| Deletion Size | 19,457 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 29,282,746 | 29,282,763 | ACCCGAGCCAGTGCTTTC |
| TUP | 29,282,767 | 29,282,788 | AGCGAACAGCTGGCGCTCACTC |
| TUR | 29,282,789 | 29,282,809 | CACCAGCCGAATGTGATGAAC |
| TDF | 29,301,241 | 29,301,258 | TTCGGAGAGCCCAGAGAC |
| TDP | 29,301,261 | 29,301,285 | TCAGAGTAAGAGCAGCCAGACAGCA |
| TDR | 29,301,321 | 29,301,343 | CAGCAACAGGAAAGCAATGAATG |
PCR Primers
| Primer | Sequence |
| SU | TGGATACAGTGGCCTGTCTG |
| SD | ACAGAATCAGGACCCACTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 64 bp | + | + | - |
| TDF | TDR | 103 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13217A-F6 | VGB6 | C57BL/6NTac | |
| 13217A-H10 | VGB6 | C57BL/6NTac | |
