| Gene | Exog |
| Chromosome | 9 |
| Deletion Start | 119,444,991 |
| Deletion End | 119,462,850 |
| Deletion Size | 17,860 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 119,445,846 | 119,445,866 | AGAAGTGAGCACAGACCCATC |
| TUP | 119,445,867 | 119,445,891 | TCATTCCAGGACGTGCTGAACCACC |
| TUR | 119,445,911 | 119,445,931 | GAGCAGTCCTCTGGCACAATG |
| TDF | 119,462,624 | 119,462,642 | CTGCAAGCTCCTGGGTTTC |
| TDP | 119,462,643 | 119,462,670 | CAGGAATTCACTCTGTACCTGAGCACGA |
| TDR | 119,462,675 | 119,462,692 | GACCGGGCTCCATCAATC |
PCR Primers
| Primer | Sequence |
| SU | GGAGATCTTTGATGGATCATG |
| SD | GTCTTGACTCTGAGGCACCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13222A-C7 | VGB6 | C57BL/6NTac | |
| 13222A-C9 | VGB6 | C57BL/6NTac | |
| 13222A-F7 | VGB6 | C57BL/6NTac | |
| 13222A-H12 | VGB6 | C57BL/6NTac | |
