| Gene | Cnnm4 |
| Chromosome | 1 |
| Deletion Start | 36,472,077 |
| Deletion End | 36,506,709 |
| Deletion Size | 34,633 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 36,472,166 | 36,472,184 | CAGATGGACGGACAAGGAC |
| TUP | 36,472,185 | 36,472,207 | TCCCTGCTCTTCATGGTGGAGGA |
| TUR | 36,472,227 | 36,472,247 | GAGAAGGATGTGCAGCCAGAG |
| TDF | 36,505,834 | 36,505,853 | TCTGTTCTGGAGGCAGTGTG |
| TDP | 36,505,860 | 36,505,884 | CCAAACCCGAGTCTCTTACCTGCCA |
| TDR | 36,505,891 | 36,505,914 | GGAATGTAGTTCTGTGGTGAGGTG |
PCR Primers
| Primer | Sequence |
| SU | GCTAGCGAGCTGCAACAAG |
| SD | GAGTGACAGACACAGGCTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13224A-A3 | VGB6 | C57BL/6NTac | |
| 13224A-C4 | VGB6 | C57BL/6NTac | |
| 13224A-B9 | VGB6 | C57BL/6NTac | |
| 13224A-A9 | VGB6 | C57BL/6NTac | |
