| Gene | Dner |
| Chromosome | 1 |
| Deletion Start | 84,695,725 |
| Deletion End | 84,695,458 |
| Deletion Size | 268 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | CGTGCTGGCCCTGCTG |
| TUP | 84,695,649 | 84,695,668 | CCCCTGCTGCTGGGTGCTGG |
| TUR | | | GCGGACACCGGGCTA |
| TDF | 84,695,525 | 84,695,543 | TTCGGTGACCGACCAAGAG |
| TDP | 84,695,504 | 84,695,524 | CACCCTGCGGCCGATGCTGAG |
| TDR | 84,695,468 | 84,695,486 | AGAGACCCCAGCGGGACAG |
PCR Primers
| Primer | Sequence |
| SU | CCAGCATCACCTCTCTCTCC |
| SD | ATGACAGATCCACGAGTACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13226A-C9 | VGB6 | C57BL/6NTac | |
