| Gene | Usp48 |
| Chromosome | 4 |
| Deletion Start | 137,594,386 |
| Deletion End | 137,623,384 |
| Deletion Size | 28,999 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 137,594,695 | 137,594,717 | GCTTAGTAAGTAACGCTGGAGTG |
| TUP | 137,594,721 | 137,594,745 | TTTGATCTTGGGCATAGCTGTGCGC |
| TUR | 137,594,764 | 137,594,782 | GTTCGCTGTCGGCAAGTTC |
| TDF | 137,623,241 | 137,623,262 | TGACGGCGTTTGTTTCTGATAC |
| TDP | 137,623,268 | 137,623,297 | CTCTAGTGAAAGCCCTTTGTAAGGACTGCG |
| TDR | 137,623,313 | 137,623,331 | TTCTTCAGGCGCAGGACAC |
PCR Primers
| Primer | Sequence |
| SU | GTCGGATTACATGGGCTTTC |
| SD | GCACACATATCCACTAACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13228A-A4 | VGB6 | C57BL/6NTac | |
| 13228A-B6 | VGB6 | C57BL/6NTac | |
| 13228A-G9 | VGB6 | C57BL/6NTac | |
| 13228A-E3 | VGB6 | C57BL/6NTac | |
| 13228A-F8 | VGB6 | C57BL/6NTac | |
