| Gene | Vasn |
| Chromosome | 16 |
| Deletion Start | 4,648,194 |
| Deletion End | 4,650,212 |
| Deletion Size | 2,019 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,648,282 | 4,648,299 | GCAACCAGCCACAGACAG |
| TUP | 4,648,300 | 4,648,321 | TCTTCTGCACTGCCCGTCAGGG |
| TUR | 4,648,370 | 4,648,390 | GTCGTGATGCCGTTCTCAAAG |
| TDF | 4,649,441 | 4,649,459 | TGGTGGTAGCTGCCGTTTG |
| TDP | 4,649,462 | 4,649,484 | AGCAAGACACCACTGGGAGTGCC |
| TDR | 4,649,517 | 4,649,536 | CCTTGCTCCACTGGACTCTC |
PCR Primers
| Primer | Sequence |
| SU | TCAGGACCCACAGTTCTTCC |
| SD | CAGAGCACCTTCCTTTCCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13229A-A3 | VGB6 | C57BL/6NTac | |
| 13229A-A6 | VGB6 | C57BL/6NTac | |
| 13229A-A9 | VGB6 | C57BL/6NTac | |
| 13229A-D8 | VGB6 | C57BL/6NTac | |
| 13229A-E8 | VGB6 | C57BL/6NTac | |
