| Gene | Mtnr1b |
| Chromosome | 9 |
| Deletion Start | 15,874,457 |
| Deletion End | 15,862,627 |
| Deletion Size | 11,831 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 15,873,803 | 15,873,823 | TGCCAAGCTGACTTCATACAC |
| TUP | 15,873,768 | 15,873,795 | TTTAAAGACCCTTGCTGGCAGAACGACC |
| TUR | 15,873,738 | 15,873,760 | CCATCCATTGCTCACGTCCATAC |
| TDF | 15,862,820 | 15,862,837 | GGGCTCCTGAACCAGAAC |
| TDP | 15,862,795 | 15,862,818 | TCCGCAGGGAGTACAAGAGGATCC |
| TDR | 15,862,728 | 15,862,751 | CGCTCCTCAGTAAGACAGTGTTTG |
PCR Primers
| Primer | Sequence |
| SU | ATTCTAAGATGGGCCAGCAC |
| SD | GTCCCTGGAAGTCACTCACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13231A-A12 | VGB6 | C57BL/6NTac | |
| 13231B-G8 | VGB6 | C57BL/6NTac | |
| 13231B-G10 | VGB6 | C57BL/6NTac | |
