| Gene | Tpcn1 |
| Chromosome | 5 |
| Deletion Start | 120,585,293 |
| Deletion End | 120,555,871 |
| Deletion Size | 29,423 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 120,584,539 | 120,584,558 | GTTAGGGAGCAGGGCTTCAG |
| TUP | 120,584,512 | 120,584,536 | CTTCCCGGTGAGTGCAGACAGCCTT |
| TUR | 120,584,477 | 120,584,497 | GTGGGCTTGTGGTATCCTTTG |
| TDF | 120,556,009 | 120,556,027 | CTAACCGTGTCCCGACCTG |
| TDP | 120,555,985 | 120,556,008 | TCATCTCCAGACGTCCGTCCTCGT |
| TDR | 120,555,958 | 120,555,978 | ACCACAATGGCCTCGATGAAC |
PCR Primers
| Primer | Sequence |
| SU | AATGTTGAAACACAGTGGCAG |
| SD | ACCCAATCCCAGGAGAGTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13234A-H10 | VGB6 | C57BL/6NTac | |
| 13234A-G2 | VGB6 | C57BL/6NTac | |
| 13234A-C6 | VGB6 | C57BL/6NTac | |
| 13234A-F7 | VGB6 | C57BL/6NTac | |
