| Gene | Sez6l2 |
| Chromosome | 7 |
| Deletion Start | 126,951,041 |
| Deletion End | 126,970,137 |
| Deletion Size | 19,097 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 126,951,747 | 126,951,767 | GAAAGCAGTCCCAAAGCACAC |
| TUP | 126,951,768 | 126,951,794 | CCTGCTAGAGCTGTCCAGGGTTAGTAC |
| TUR | 126,951,821 | 126,951,839 | AGTTCCAGCTCCTGGTCTC |
| TDF | 126,969,942 | 126,969,963 | TCCCTGGTAGTTGGGATTACAG |
| TDP | 126,969,965 | 126,969,989 | TTGGCACCACCAGACTTAGGACTGA |
| TDR | 126,969,993 | 126,970,014 | CATCCACAAGAAGCGGGAAGAC |
PCR Primers
| Primer | Sequence |
| SU | CGGTTGAACCTCATAAGGGC |
| SD | CATCCTTCTCACCCTCCAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13235A-A2 | VGB6 | C57BL/6NTac | |
| 13235A-B12 | VGB6 | C57BL/6NTac | |
| 13235A-E9 | VGB6 | C57BL/6NTac | |
| 13235A-D12 | VGB6 | C57BL/6NTac | |
| 13235A-C8 | VGB6 | C57BL/6NTac | |
| 13235A-B2 | VGB6 | C57BL/6NTac | |
