Information regarding availability, timelines and delivery of materials can be found at www.komp.org or www.knockoutmouse.org.

13238 Gpr171 Status: ES cell clone microinjected

Gene	Gpr171
Chromosome	3
Deletion Start	59,098,349
Deletion End	59,097,392
Deletion Size	958
Genome Build	38

MGI ID	MGI:2442043
NCBI Gene ID	229323
BAC ID	261F18
BAC Library	bMQ
Cassette	ZEN-Ub1
Clones Available	Yes

Sequence Downloads and Strategy Information

Primer Genomic Coordinates on Chromosome 3

Please be aware that these oligonucleotides have been validated only by Taqman assay.

Primer	Start	End	Sequence
TUF	59,098,105	59,098,123	TGGCACCCTGGAAGCTAAG
TUP	59,098,077	59,098,100	TTCCACTGCCAAGTGACAGCCTGC
TUR	59,098,022	59,098,044	CGATGCTGACAAAGGCTAAGAAG
TDF	59,097,627	59,097,645	TGTTCCCTACCACGCTGTC
TDP	59,097,600	59,097,626	AGGATCCCATACACCCTCAGCCAGACA
TDR	59,097,560	59,097,582	TGAAGAGTGCGATCCTAGTTGAG

PCR Primers

Primer	Sequence
SU	CGCAAAGAAATGTCTCCCTC
SD	TTAAGGTATCGCTGAAGGGC
LacInR	TTGACTGTAGCGGCTGATGTTG
LacInf	GGTAAACTGGCTCGGATTAGGG
NeoInR	TACTTTCTCGGCAGGAGCAAGGTG
NeoInF	TTCGGCTATGACTGGGCACAACAG
LacInZRev	GTCTGTCCTAGCTTCCTCACTG
NeoFwd	TCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward Primer	Reverse Primer	Length	WT	HET	KO
SU	LacZRev	bp	-	+	+
TUF	TUR	102 bp	+	+	-
TDF	TDR	86 bp	+	+	-
NeoFwd	SD	bp	-	+	+
LacInf	LacInR	210 bp	-	+	+
NeoInf	NeoInR	282 bp	-	+	+

Available Clones

ES Cell ordering information can be found at the KOMP Repository.

Clone Name	Parental ES Cell	Mouse Strain	Cassette Copy Number
13238A-C11	VGB6	C57BL/6NTac
13238A-E8	VGB6	C57BL/6NTac
13238A-D8	VGB6	C57BL/6NTac
13238A-A7	VGB6	C57BL/6NTac