| Gene | Dnajc16 |
| Chromosome | 4 |
| Deletion Start | 141,789,052 |
| Deletion End | 141,763,518 |
| Deletion Size | 25,535 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 141,788,843 | 141,788,862 | AACGTCCGCTAGGCAGTCAG |
| TUP | 141,788,809 | 141,788,834 | TGAAGCCTCAATGAGGGTGACTTGTC |
| TUR | 141,788,770 | 141,788,794 | ACTCTAGGCGGTTCTACAAATACAC |
| TDF | 141,763,861 | 141,763,882 | CCACTTCTCCTTCCTGACACTG |
| TDP | 141,763,834 | 141,763,859 | ACAAACACCGGGAGTGGCTGGAGTAT |
| TDR | 141,763,808 | 141,763,825 | GCCGCATCCTGAGCAAAC |
PCR Primers
| Primer | Sequence |
| SU | CACCACTTGTTTCAGAGCATTC |
| SD | GTTGAGAGCACACACCTTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13239A-E6 | VGB6 | C57BL/6NTac | |
| 13239A-G3 | VGB6 | C57BL/6NTac | |
| 13239A-G11 | VGB6 | C57BL/6NTac | |
| 13239A-H3 | VGB6 | C57BL/6NTac | |
| 13239A-H8 | VGB6 | C57BL/6NTac | |
