| Gene | Dhrs9 |
| Chromosome | 2 |
| Deletion Start | 69,392,913 |
| Deletion End | 69,401,489 |
| Deletion Size | 8,577 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 69,393,415 | 69,393,434 | AGGGATGTGCCCAGATAGAC |
| TUP | 69,393,438 | 69,393,462 | CCTTGAGTCCCAGCAGGTGGATCTA |
| TUR | 69,393,463 | 69,393,483 | TCAGGACCTTTATGCCTGTTC |
| TDF | 69,401,302 | 69,401,325 | GACCACGCTCTAACAAGTCTCTTC |
| TDP | 69,401,326 | 69,401,350 | CCCAAGACTCGGTACATTGCTGGGA |
| TDR | 69,401,363 | 69,401,386 | TGTGAGACAGAGGTATCCAGAATG |
PCR Primers
| Primer | Sequence |
| SU | GATGGATGAGTTTGCGTGTG |
| SD | GGGCAGGTAGCAGCACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13240A-A1 | VGB6 | C57BL/6NTac | |
| 13240A-A2 | VGB6 | C57BL/6NTac | |
| 13240A-D2 | VGB6 | C57BL/6NTac | |
| 13240A-F11 | VGB6 | C57BL/6NTac | |
| 13240A-D10 | VGB6 | C57BL/6NTac | |
| 13240A-F3 | VGB6 | C57BL/6NTac | |
