| Gene | Tmem203 |
| Chromosome | 2 |
| Deletion Start | 25,255,673 |
| Deletion End | 25,256,156 |
| Deletion Size | 484 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,255,703 | 25,255,721 | CTGGGCTTCGCCACCTTTG |
| TUP | 25,255,722 | 25,255,746 | AGATATTCGTGCACCTGCTGGCCCT |
| TUR | 25,255,763 | 25,255,780 | CACTCGCAGTGCCAACAG |
| TDF | 25,256,020 | 25,256,041 | CACGTCTCCGGTCTTCATTCTC |
| TDP | 25,256,045 | 25,256,069 | CAGCTGCTCATGATCCGGGCTTGTC |
| TDR | 25,256,096 | 25,256,113 | GCGCAGTGCTCCATTTCC |
PCR Primers
| Primer | Sequence |
| SU | AACCTGCATTACTGCACACG |
| SD | CTCTGAGGTCAGTGCTGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13243A-E3 | VGB6 | C57BL/6NTac | |
| 13243A-E11 | VGB6 | C57BL/6NTac | |
| 13243A-F3 | VGB6 | C57BL/6NTac | |
| 13243A-E7 | VGB6 | C57BL/6NTac | |
| 13243A-D5 | VGB6 | C57BL/6NTac | |
