Gene | Frem2 |
Chromosome | 3 |
Deletion Start | 53,657,081 |
Deletion End | 53,647,890 |
Deletion Size | 9,192 |
Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
Primer | Start | End | Sequence |
TUF | 53,656,459 | 53,656,478 | CAGCCTGAGACCGAGGAGTG |
TUP | 53,656,435 | 53,656,457 | CGCGTGGGCATCTTGTCAGGTCT |
TUR | 53,656,386 | 53,656,406 | CTGGACCTGTGGGTAGTGAAG |
TDF | 53,648,335 | 53,648,356 | AAACTCACTCTGCAAGGGACAC |
TDP | 53,648,299 | 53,648,325 | TGAAGAGGTCCAGGAAATCTGCCATGC |
TDR | 53,648,262 | 53,648,284 | CAGGCTCTATGACAGGTGGAAAG |
PCR Primers
Primer | Sequence |
SU | ACCAACCTGTTGCACCTCTC |
SD | TAGGAGACCTAGGTAGGAAG |
LacInR | TTGACTGTAGCGGCTGATGTTG |
LacInf | GGTAAACTGGCTCGGATTAGGG |
NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
NeoInF | TTCGGCTATGACTGGGCACAACAG |
LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
Forward Primer | Reverse Primer | Length | WT | HET | KO |
SU | LacZRev | bp | - | + | + |
TUF | TUR | 93 bp | + | + | - |
TDF | TDR | 95 bp | + | + | - |
NeoFwd | SD | bp | - | + | + |
LacInf | LacInR | 210 bp | - | + | + |
NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
13246A-C5 | VGB6 | C57BL/6NTac | |
13246A-F4 | VGB6 | C57BL/6NTac | |
13246A-G8 | VGB6 | C57BL/6NTac | |
13246A-G12 | VGB6 | C57BL/6NTac | |