| Gene | Frem2 |
| Chromosome | 3 |
| Deletion Start | 53,657,081 |
| Deletion End | 53,647,890 |
| Deletion Size | 9,192 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 53,656,459 | 53,656,478 | CAGCCTGAGACCGAGGAGTG |
| TUP | 53,656,435 | 53,656,457 | CGCGTGGGCATCTTGTCAGGTCT |
| TUR | 53,656,386 | 53,656,406 | CTGGACCTGTGGGTAGTGAAG |
| TDF | 53,648,335 | 53,648,356 | AAACTCACTCTGCAAGGGACAC |
| TDP | 53,648,299 | 53,648,325 | TGAAGAGGTCCAGGAAATCTGCCATGC |
| TDR | 53,648,262 | 53,648,284 | CAGGCTCTATGACAGGTGGAAAG |
PCR Primers
| Primer | Sequence |
| SU | ACCAACCTGTTGCACCTCTC |
| SD | TAGGAGACCTAGGTAGGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13246A-C5 | VGB6 | C57BL/6NTac | |
| 13246A-F4 | VGB6 | C57BL/6NTac | |
| 13246A-G8 | VGB6 | C57BL/6NTac | |
| 13246A-G12 | VGB6 | C57BL/6NTac | |
