13247   Pptc7     Status: ES cell colonies screened / QC positives

GenePptc7
Chromosome5
Deletion Start122,284,672
Deletion End122,320,949
Deletion Size36,278
Genome Build38
MGI IDMGI:2444593
NCBI Gene ID320717
BAC ID184M9
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 5

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF122,285,210122,285,230CAGGCTGCTAACCTCGCTTTG
TUP122,285,231122,285,254CCTCACTCCCTCCTCCCTCTTGGC
TUR122,285,262122,285,282CTCGCCAAGAAGCAGGGATTC
TDF122,320,373122,320,390TAGCCGTCCTGCACTTAC
TDP122,320,391122,320,413TCATGTGATGCTGTGGGCCGACC
TDR122,320,423122,320,442ACCCTGCCGCTTTCCTACTC

PCR Primers

PrimerSequence
SUAGTGTGGTTGAGGCGGAG
SDCCGAGGTGCCGCTATTTAC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR73 bp++-
TDFTDR70 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
13247A-B6VGB6C57BL/6NTac
13247A-B8VGB6C57BL/6NTac
13247A-C9VGB6C57BL/6NTac
13247A-D4VGB6C57BL/6NTac
13247A-H12VGB6C57BL/6NTac
13247A-E9VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
Larger Image

VelociGene Genotyping Strategy

Larger Image