| Gene | Otos |
| Chromosome | 1 |
| Deletion Start | 92,645,388 |
| Deletion End | 92,644,232 |
| Deletion Size | 1,157 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 92,644,880 | 92,644,899 | GGAGTCCCAAGACCACAGAG |
| TUP | 92,644,845 | 92,644,870 | CCTGTGCTCTAGAACGTGGGTGCTGT |
| TUR | 92,644,822 | 92,644,841 | ATGCCCACGAGGCTTCAGAG |
| TDF | 92,644,667 | 92,644,688 | AACGGGTAAGAGGTGCATTCAG |
| TDP | 92,644,639 | 92,644,664 | TGACTCAGACTCTACTCCTGGCTGGG |
| TDR | 92,644,600 | 92,644,618 | GGATGGTGCTTCCAGTGTC |
PCR Primers
| Primer | Sequence |
| SU | GTGACGCTTCCTTCCTTGTC |
| SD | ACTCCCAGGAACATGCTGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13256A-A1 | VGB6 | C57BL/6NTac | |
| 13256A-A10 | VGB6 | C57BL/6NTac | |
| 13256A-B11 | VGB6 | C57BL/6NTac | |
| 13256A-F7 | VGB6 | C57BL/6NTac | |
| 13256A-G2 | VGB6 | C57BL/6NTac | |
| 13256A-G3 | VGB6 | C57BL/6NTac | |
