| Gene | Gm1123 |
| Chromosome | 9 |
| Deletion Start | 99,035,620 |
| Deletion End | 99,006,992 |
| Deletion Size | 28,629 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 99,035,323 | 99,035,346 | CCATGTTAGCTTGTGCTTAGTGTC |
| TUP | 99,035,286 | 99,035,313 | ATTGCAAATGCTTGGTCAGGTACGGGCT |
| TUR | 99,035,250 | 99,035,271 | GGTTGGGAGTCCAGTGAGTTAG |
| TDF | 99,007,237 | 99,007,256 | TGTAGCCCTCGCTGATCTTG |
| TDP | 99,007,210 | 99,007,234 | CTTAGGCTCCCTCTGCCTTTCCTCC |
| TDR | 99,007,158 | 99,007,181 | GCCTCAACAATGCAAACCACTTAC |
PCR Primers
| Primer | Sequence |
| SU | GTGGTGCAAGTTGTCTGTGC |
| SD | GAGCCACTGCTGTGATGAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13261A-B5 | VGB6 | C57BL/6NTac | |
| 13261A-B6 | VGB6 | C57BL/6NTac | |
| 13261A-F7 | VGB6 | C57BL/6NTac | |
| 13261A-H9 | VGB6 | C57BL/6NTac | |
