| Gene | Cd28 |
| Chromosome | 1 |
| Deletion Start | 60,746,477 |
| Deletion End | 60,769,834 |
| Deletion Size | 23,358 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 60,747,009 | 60,747,032 | GCCTGGGCTCATATCTCAATTCTC |
| TUP | 60,747,038 | 60,747,063 | TGGCGTTTGGAGCAGGATTTACTACC |
| TUR | 60,747,133 | 60,747,157 | AGCCTGAACTAAGACCTCCACAATG |
| TDF | 60,768,856 | 60,768,878 | CCTGGATAGTATTCCCAGCAGTC |
| TDP | 60,768,880 | 60,768,906 | TTGCTTACTTTGAGGCCTTGGCATGTG |
| TDR | 60,768,949 | 60,768,969 | GGGACAGGTGTTCCTGGATTG |
PCR Primers
| Primer | Sequence |
| SU | CACATTTCACAGCTCCGATG |
| SD | CATGACTGAAATGTGCTGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 149 bp | + | + | - |
| TDF | TDR | 114 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13277A-A3 | VGB6 | C57BL/6NTac | |
| 13277A-A5 | VGB6 | C57BL/6NTac | |
| 13277A-E3 | VGB6 | C57BL/6NTac | |
| 13277A-G11 | VGB6 | C57BL/6NTac | |
| 13277A-G7 | VGB6 | C57BL/6NTac | |
| 13277A-E2 | VGB6 | C57BL/6NTac | |
