| Gene | Gjb2 |
| Chromosome | 14 |
| Deletion Start | 57,100,746 |
| Deletion End | 57,100,059 |
| Deletion Size | 688 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 57,100,657 | 57,100,678 | GCTCACGGTCCTCTTCATCTTC |
| TUP | 57,100,630 | 57,100,652 | TCATGATCCTCGTGGTGGCTGCA |
| TUR | 57,100,546 | 57,100,567 | GTGGTGGTCGTAGCATACATTC |
| TDF | 57,100,327 | 57,100,347 | ACCTACACCACCAGCATCTTC |
| TDP | 57,100,303 | 57,100,325 | TCCGGGTCATCTTTGAAGCCGTC |
| TDR | 57,100,232 | 57,100,251 | GGCCAAGCGTTGCATTTCAC |
PCR Primers
| Primer | Sequence |
| SU | CAGAAATGCGAACATATGGG |
| SD | CTTTCTCAGTGGCACCTTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 133 bp | + | + | - |
| TDF | TDR | 116 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13295A-C12 | VGB6 | C57BL/6NTac | |
| 13295A-E3 | VGB6 | C57BL/6NTac | |
| 13295A-A7 | VGB6 | C57BL/6NTac | |
| 13295A-A8 | VGB6 | C57BL/6NTac | |
| 13295A-B5 | VGB6 | C57BL/6NTac | |
