| Gene | Lrp2 |
| Chromosome | 2 |
| Deletion Start | 69,552,452 |
| Deletion End | 69,521,724 |
| Deletion Size | 30,729 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 69,552,423 | 69,552,441 | TCGCTGTGACAACGGATAC |
| TUP | 69,552,394 | 69,552,418 | TCCCTGCATCCTGGAGGTGTGATGG |
| TUR | 69,552,332 | 69,552,352 | GCCTGGCTCCTAGTCACTTAC |
| TDF | 69,521,810 | 69,521,828 | GGCGGTGATGAAGGCAAAC |
| TDP | 69,521,779 | 69,521,806 | TTCACAGACACCAACCCTCAAGTGTACC |
| TDR | 69,521,729 | 69,521,750 | CCTGAGGGCATGGTAAACTGTC |
PCR Primers
| Primer | Sequence |
| SU | TCTCACATTCTGATCATGGTC |
| SD | ACTAGAACCAAGCACCTGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13296A-G5 | VGB6 | C57BL/6NTac | |
| 13296A-F3 | VGB6 | C57BL/6NTac | |
| 13296A-E7 | VGB6 | C57BL/6NTac | |
| 13296A-C7 | VGB6 | C57BL/6NTac | |
| 13296A-A7 | VGB6 | C57BL/6NTac | |
