13297   Grpr     Status: ES cell colonies screened / QC positives

GeneGrpr
ChromosomeX
Deletion Start163,549,294
Deletion End163,514,834
Deletion Size34,461
Genome Build38
MGI IDMGI:95836
NCBI Gene ID14829
BAC ID417C5
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome X

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF163,549,106163,549,126GGCAACATCACGCTCATCAAG
TUP163,549,080163,549,104TCTTCTGCACGGTCAAGTCCATGCG
TUR163,549,039163,549,058CTCCCAAAGCCAGGCTAGAG
TDF163,515,169163,515,190TGTACCGTTCCTACCACTACTC
TDP163,515,145163,515,168TGAAGTGGACACCTCCATGCTCCA
TDR163,515,124163,515,144GCACAGATGCTGGTGACAAAG

PCR Primers

PrimerSequence
SUTACAAATCAGGGAAGACGGG
SDTGACCATACTTGCCTAGTGGG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR88 bp++-
TDFTDR67 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
13297B-A10VGB6C57BL/6NTac
13297B-F2VGB6C57BL/6NTac
13297B-F4VGB6C57BL/6NTac
13297B-F12VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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