| Gene | Gypa |
| Chromosome | 8 |
| Deletion Start | 80,494,057 |
| Deletion End | 80,509,541 |
| Deletion Size | 15,485 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 80,494,232 | 80,494,252 | GGATTCAGCTAGGGACAGGAG |
| TUP | 80,494,253 | 80,494,273 | TGGCACACTGCAGCCCACAAC |
| TUR | 80,494,274 | 80,494,297 | GGAACTGCTATTCAGGCACAAATG |
| TDF | 80,508,989 | 80,509,008 | GTCTGGGAGACCGTCTTGTG |
| TDP | 80,509,010 | 80,509,035 | CACCAAACTCAGCTTCTCAGCAGGCA |
| TDR | 80,509,062 | 80,509,084 | GGTAGGCTGTCAAGTCTGTGATG |
PCR Primers
| Primer | Sequence |
| SU | CCTACTCTCCAAGTCCAGCG |
| SD | TAAGAGAGCTGTGTGCTGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 66 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13298A-A1 | VGB6 | C57BL/6NTac | |
| 13298A-B3 | VGB6 | C57BL/6NTac | |
| 13298A-E8 | VGB6 | C57BL/6NTac | |
| 13298A-F7 | VGB6 | C57BL/6NTac | |
| 13298A-H11 | VGB6 | C57BL/6NTac | |
