| Gene | Htr2c |
| Chromosome | X |
| Deletion Start | 147,072,456 |
| Deletion End | 147,076,043 |
| Deletion Size | 3,588 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 147,072,809 | 147,072,831 | TGTGGTGCTATAAACCTGTCGTC |
| TUP | 147,072,837 | 147,072,864 | CCCAAGGTGACTGAGACAGGGTGGTTAC |
| TUR | 147,072,884 | 147,072,905 | TCCAGTTCAGCGTGTATGTATC |
| TDF | 147,075,744 | 147,075,764 | GGCCTATTGGTTTGGCAGTTC |
| TDP | 147,075,770 | 147,075,787 | TTCCATAAGTCCAGTAGC |
| TDR | 147,075,803 | 147,075,826 | CCACCATCGGAGGAATTAAAAGTG |
PCR Primers
| Primer | Sequence |
| SU | CAAGGGAATATGCTGTAAAGGC |
| SD | TGCCATTTTGCCACTATCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13299A-E6 | VGB6 | C57BL/6NTac | |
| 13299B-F9 | VGB6 | C57BL/6NTac | |
