| Gene | Itga1 |
| Chromosome | 13 |
| Deletion Start | 115,049,377 |
| Deletion End | 115,030,042 |
| Deletion Size | 19,336 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 115,049,338 | 115,049,358 | CCATGAGCTTCAGTGGTCCAG |
| TUP | 115,049,310 | 115,049,337 | TGGAGGACATGTTTGGATACACCGTTCA |
| TUR | 115,049,272 | 115,049,293 | TGTTTGCTTACCATTTGCCTTC |
| TDF | 115,030,835 | 115,030,859 | TGCATGTTTGAAACCATAAGCAATG |
| TDP | 115,030,803 | 115,030,832 | TGAGCTACATGTCTACCCAAGAGTACACCA |
| TDR | 115,030,750 | 115,030,770 | GTCAAGCGTTTGCCTAAGTGG |
PCR Primers
| Primer | Sequence |
| SU | TGTGCAAACTCATGAGTCATC |
| SD | TAGTAGGTCTGGATATGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13301A-C5 | VGB6 | C57BL/6NTac | |
| 13301A-F10 | VGB6 | C57BL/6NTac | |
| 13301A-C12 | VGB6 | C57BL/6NTac | |
| 13301A-G7 | VGB6 | C57BL/6NTac | |
| 13301A-H2 | VGB6 | C57BL/6NTac | |
| 13301A-A1 | VGB6 | C57BL/6NTac | |
