| Gene | Kcna2 |
| Chromosome | 3 |
| Deletion Start | 107,104,108 |
| Deletion End | 107,105,630 |
| Deletion Size | 1,523 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 107,104,453 | 107,104,476 | GAGCTAGGAGAAGAAGCAATGGAG |
| TUP | 107,104,478 | 107,104,505 | TGTTTCGGGAGGATGAAGGCTACATCAA |
| TUR | 107,104,536 | 107,104,555 | GCCACACCTGTCTCTGAAAC |
| TDF | 107,104,925 | 107,104,944 | TAGCTGAGAAGCCAGAGGAC |
| TDP | 107,104,948 | 107,104,969 | CAGCAAGGCCAGCAGGCCATGT |
| TDR | 107,105,022 | 107,105,042 | CCTTTGGAGTGTCTGGACAAC |
PCR Primers
| Primer | Sequence |
| SU | CTTCTCCACTCCTCCTGTGC |
| SD | AGATGGGCTGAAATAGGGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 118 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13302A-A8 | VGB6 | C57BL/6NTac | |
| 13302A-A12 | VGB6 | C57BL/6NTac | |
| 13302A-B7 | VGB6 | C57BL/6NTac | |
| 13302A-F8 | VGB6 | C57BL/6NTac | |
| 13302A-E1 | VGB6 | C57BL/6NTac | |
| 13302A-B10 | VGB6 | C57BL/6NTac | |
