| Gene | Kcne1 |
| Chromosome | 16 |
| Deletion Start | 92,348,951 |
| Deletion End | 92,348,551 |
| Deletion Size | 401 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 92,348,852 | 92,348,870 | GGCCTGGCTCGTAAGTCTC |
| TUP | 92,348,824 | 92,348,848 | TCCGAGATGACAGCAAGCTAGAGGC |
| TUR | 92,348,778 | 92,348,799 | CAGGGTGAAGAAGCCGAAGAAG |
| TDF | 92,348,721 | 92,348,740 | AGCACTCCCACGACCCTTTC |
| TDP | 92,348,692 | 92,348,716 | TGTACATCGAGTCAGATGCCTGGCA |
| TDR | 92,348,670 | 92,348,688 | GAAGACGGCCTTGCCTTTC |
PCR Primers
| Primer | Sequence |
| SU | AATGGGCCGTAAAGTCAATG |
| SD | TACAAAGACAAGGACAGGCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13303A-B2 | VGB6 | C57BL/6NTac | |
| 13303A-B4 | VGB6 | C57BL/6NTac | |
| 13303A-B8 | VGB6 | C57BL/6NTac | |
| 13303A-C6 | VGB6 | C57BL/6NTac | |
| 13303A-E11 | VGB6 | C57BL/6NTac | |
| 13303A-H8 | VGB6 | C57BL/6NTac | |
