| Gene | Ldlr |
| Chromosome | 9 |
| Deletion Start | 21,723,781 |
| Deletion End | 21,748,279 |
| Deletion Size | 24,499 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 21,724,434 | 21,724,455 | GTCCCTACCTTGTCCCTACTTC |
| TUP | 21,724,458 | 21,724,480 | TGGTTTGGTGCAGATGTGCCTCA |
| TUR | 21,724,503 | 21,724,520 | TCCCTGCCTGATGGTCTC |
| TDF | 21,748,079 | 21,748,099 | TGCCATGCTTGTGTATAGTCC |
| TDP | 21,748,107 | 21,748,134 | CTGATTGTTTCCTGTGTTTCCGCTTTGC |
| TDR | 21,748,156 | 21,748,175 | CATGCCACATCGTCCTCCAG |
PCR Primers
| Primer | Sequence |
| SU | TCTGTGGGAGGAATTTGAGG |
| SD | AAGGCTCTGGGATGAAGGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13304B-C8 | VGB6 | C57BL/6NTac | |
| 13304B-D4 | VGB6 | C57BL/6NTac | |
| 13304B-D9 | VGB6 | C57BL/6NTac | |
| 13304B-E2 | VGB6 | C57BL/6NTac | |
| 13304B-E3 | VGB6 | C57BL/6NTac | |
| 13304B-G6 | VGB6 | C57BL/6NTac | |
