| Gene | Lifr |
| Chromosome | 15 |
| Deletion Start | 7,154,412 |
| Deletion End | 7,191,196 |
| Deletion Size | 36,785 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 7,154,555 | 7,154,575 | AGCCTCAAAGCAAACGGCAAG |
| TUP | 7,154,576 | 7,154,600 | CTGGTTGGCATGCGACCGTAGAGTA |
| TUR | 7,154,606 | 7,154,628 | CCTGAAGAGGTCAGCAGAGAAAC |
| TDF | 7,190,701 | 7,190,722 | GAACCATGTGGTCGTGTCTTAC |
| TDP | 7,190,723 | 7,190,744 | TGCCCACCCATCATCGAGGAGG |
| TDR | 7,190,830 | 7,190,850 | TCCTGCTCTTCCTCTGCTTTG |
PCR Primers
| Primer | Sequence |
| SU | TCCCGTGGAGTATACTTGGG |
| SD | TCCCTTGCCTGTAAATCCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 150 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13305A-A7 | VGB6 | C57BL/6NTac | |
| 13305A-A9 | VGB6 | C57BL/6NTac | |
| 13305A-D2 | VGB6 | C57BL/6NTac | |
| 13305A-F11 | VGB6 | C57BL/6NTac | |
| 13305A-G7 | VGB6 | C57BL/6NTac | |
| 13305A-D7 | VGB6 | C57BL/6NTac | |
