| Gene | Mas1 |
| Chromosome | 17 |
| Deletion Start | 12,842,531 |
| Deletion End | 12,841,512 |
| Deletion Size | 1,020 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 12,842,485 | 12,842,508 | TGCTGAAGAGAAAGCCATGAATAC |
| TUP | 12,842,461 | 12,842,483 | TCCAGCAGAAATGCCTCCCTGGG |
| TUR | 12,842,434 | 12,842,457 | ACTATGGGAATGGGTGGGTGTGAG |
| TDF | 12,841,779 | 12,841,802 | GTCCTCTACTTGCTGTACTACGAG |
| TDP | 12,841,754 | 12,841,777 | ACTGGTCAGCCTTTGGGAACCTGC |
| TDR | 12,841,710 | 12,841,728 | GTTGGCGCTGCTGTTGATG |
PCR Primers
| Primer | Sequence |
| SU | TCAGCTGTCTCTTTCATTTCAA |
| SD | GGTGCTTTGCCTACCTCATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13306A-A5 | VGB6 | C57BL/6NTac | |
| 13306A-B3 | VGB6 | C57BL/6NTac | |
| 13306A-C1 | VGB6 | C57BL/6NTac | |
| 13306A-C4 | VGB6 | C57BL/6NTac | |
| 13306A-D3 | VGB6 | C57BL/6NTac | |
| 13306A-D7 | VGB6 | C57BL/6NTac | |
