| Gene | Mbl1 |
| Chromosome | 14 |
| Deletion Start | 41,153,594 |
| Deletion End | 41,158,885 |
| Deletion Size | 5,292 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 41,154,472 | 41,154,489 | TGGGACATGCTGCCTTTG |
| TUP | 41,154,490 | 41,154,512 | TCTCCCAACAGGTCAAGGGCTCA |
| TUR | 41,154,555 | 41,154,572 | TTCCAGGGCTTCCAACAC |
| TDF | 41,158,235 | 41,158,255 | CACAAGTGCCACCCTGTGTTC |
| TDP | 41,158,263 | 41,158,289 | TTCTGACTGTTGATGTCAGCCGTGAAC |
| TDR | 41,158,332 | 41,158,353 | GCAAGCAGTACAGCCATGAAAG |
PCR Primers
| Primer | Sequence |
| SU | AAAGAGAGATCCAGGGTCCG |
| SD | TCACGGAGAAAGGAAATTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 119 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13307A-A9 | VGB6 | C57BL/6NTac | |
| 13307A-C9 | VGB6 | C57BL/6NTac | |
| 13307A-E6 | VGB6 | C57BL/6NTac | |
| 13307A-G11 | VGB6 | C57BL/6NTac | |
| 13307A-H1 | VGB6 | C57BL/6NTac | |
| 13307A-E10 | VGB6 | C57BL/6NTac | |
