| Gene | Mcpt1 |
| Chromosome | 14 |
| Deletion Start | 56,018,002 |
| Deletion End | 56,020,257 |
| Deletion Size | 2,256 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 56,018,587 | 56,018,607 | AGACCACACTCCCGTCCTTAC |
| TUP | 56,018,608 | 56,018,635 | ATGGCCCATCTGAAGATCATCACGGACA |
| TUR | 56,018,636 | 56,018,657 | CCACATCTGTCCTCAGAACCTC |
| TDF | 56,019,740 | 56,019,764 | CTCCCTTCTTTCTCAACTCCCTATG |
| TDP | 56,019,767 | 56,019,791 | CAAGCCCTGCTCACAACCTTCCCAT |
| TDR | 56,019,806 | 56,019,827 | CTCCCTTTACTCCCATCCTTTC |
PCR Primers
| Primer | Sequence |
| SU | ATGTCCTCTCCTCCATGTGC |
| SD | GAGAAGGCAGGACAGTGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13308A-A9 | VGB6 | C57BL/6NTac | |
| 13308A-B1 | VGB6 | C57BL/6NTac | |
| 13308A-C6 | VGB6 | C57BL/6NTac | |
| 13308A-E9 | VGB6 | C57BL/6NTac | |
| 13308A-D6 | VGB6 | C57BL/6NTac | |
| 13308A-G7 | VGB6 | C57BL/6NTac | |
