| Gene | Oprk1 |
| Chromosome | 1 |
| Deletion Start | 5,589,052 |
| Deletion End | 5,602,861 |
| Deletion Size | 13,810 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 5,589,236 | 5,589,259 | CCGCTGTCTACTCTGTGGTATTTG |
| TUP | 5,589,260 | 5,589,284 | TGGTGGGCTTAGTGGGCAATTCTCT |
| TUR | 5,589,297 | 5,589,317 | CCCTGAACTCACCGGATGATG |
| TDF | 5,602,524 | 5,602,543 | CTGGTGGAGGCTCTGGGAAG |
| TDP | 5,602,548 | 5,602,567 | TCCCACAGCACAGCTGCCCT |
| TDR | 5,602,603 | 5,602,621 | GGATTCAGGCTGCTGTTGG |
PCR Primers
| Primer | Sequence |
| SU | ATCAGCGATCTGGAGGTGAG |
| SD | CCCTTGATATGGCCTCATTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13309A-B3 | VGB6 | C57BL/6NTac | |
| 13309A-C10 | VGB6 | C57BL/6NTac | |
| 13309A-C12 | VGB6 | C57BL/6NTac | |
| 13309A-D1 | VGB6 | C57BL/6NTac | |
| 13309A-H8 | VGB6 | C57BL/6NTac | |
| 13309A-D7 | VGB6 | C57BL/6NTac | |
