| Gene | Phb |
| Chromosome | 11 |
| Deletion Start | 95,668,067 |
| Deletion End | 95,679,935 |
| Deletion Size | 11,869 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 95,668,125 | 95,668,144 | GCGTGGTGAACTCTGCTTTG |
| TUP | 95,668,155 | 95,668,176 | AGGCGCGGAAGAGCTTTCTGCA |
| TUR | 95,668,185 | 95,668,207 | ACACATGGGCTATGCAGATCAAC |
| TDF | 95,679,274 | 95,679,293 | GGCTTGCTTCAGGCTTTCAG |
| TDP | 95,679,296 | 95,679,315 | AGCCGCCCATCTGCCATCGC |
| TDR | 95,679,337 | 95,679,356 | ATCCCGGTGTGGTGGCTAAC |
PCR Primers
| Primer | Sequence |
| SU | GTTCCTCGTGGGTACAAAGC |
| SD | AATGGCTGGAAACACTGAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13311A-H12 | VGB6 | C57BL/6NTac | |
| 13311A-B3 | VGB6 | C57BL/6NTac | |
| 13311A-D4 | VGB6 | C57BL/6NTac | |
| 13311A-H2 | VGB6 | C57BL/6NTac | |
| 13311A-E5 | VGB6 | C57BL/6NTac | |
| 13311A-D5 | VGB6 | C57BL/6NTac | |
