| Gene | Ptger2 |
| Chromosome | 14 |
| Deletion Start | 44,988,968 |
| Deletion End | 45,002,034 |
| Deletion Size | 13,067 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 44,989,537 | 44,989,555 | GCACGGGAGGACTGCATAC |
| TUP | 44,989,559 | 44,989,581 | CAGCTGTACGCCACGATGCTCCT |
| TUR | 44,989,612 | 44,989,633 | GAGGTTGAGGATAACGCTGATG |
| TDF | 45,001,839 | 45,001,862 | CCCTTGGGTCTTTGCCATACTTAG |
| TDP | 45,001,865 | 45,001,887 | CACCGGTCCTGAGGTTAATGCGC |
| TDR | 45,001,916 | 45,001,937 | GTTTGCTGAGCTTCTTGTGTTC |
PCR Primers
| Primer | Sequence |
| SU | TTCCACTACTGGACTTGCCC |
| SD | CAGCAACTTGTCTGCTCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13313A-B12 | VGB6 | C57BL/6NTac | |
| 13313A-C4 | VGB6 | C57BL/6NTac | |
| 13313A-C10 | VGB6 | C57BL/6NTac | |
| 13313A-D7 | VGB6 | C57BL/6NTac | |
| 13313A-F3 | VGB6 | C57BL/6NTac | |
