| Gene | Ptger3 |
| Chromosome | 3 |
| Deletion Start | 157,567,021 |
| Deletion End | 157,567,839 |
| Deletion Size | 819 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 157,567,049 | 157,567,066 | CTGAAGCGCACAGCAACC |
| TUP | 157,567,069 | 157,567,093 | TCAAGTACTACCGACGACTGCGGCT |
| TUR | 157,567,122 | 157,567,141 | CCACGAAGCCAGTGACCATC |
| TDF | 157,567,668 | 157,567,687 | GGCTCTGGTGGTGACCTTTG |
| TDP | 157,567,690 | 157,567,712 | TGCAACCTGGCGACCATCAAAGC |
| TDR | 157,567,783 | 157,567,802 | ATGAGCTGGATGGCCGTCTC |
PCR Primers
| Primer | Sequence |
| SU | GTGTCACGTAACCCAAAGGC |
| SD | TCAGTCTGCACTGGCTTGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 135 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13314A-C10 | VGB6 | C57BL/6NTac | |
| 13314A-E12 | VGB6 | C57BL/6NTac | |
| 13314A-F1 | VGB6 | C57BL/6NTac | |
| 13314A-G5 | VGB6 | C57BL/6NTac | |
| 13314A-H5 | VGB6 | C57BL/6NTac | |
| 13314A-H12 | VGB6 | C57BL/6NTac | |
