| Gene | Ptprcap |
| Chromosome | 19 |
| Deletion Start | 4,154,704 |
| Deletion End | 4,156,600 |
| Deletion Size | 1,897 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,154,888 | 4,154,906 | CCTGGGCACAGCCCTATTC |
| TUP | 4,154,908 | 4,154,932 | AAGACCCTTGGGACAGTCCTCCACC |
| TUR | 4,154,966 | 4,154,988 | AGCATACCAAGCTCTGGAGAAAG |
| TDF | 4,155,669 | 4,155,690 | TCACCATGAGGCTGTGAAGTAG |
| TDP | 4,155,691 | 4,155,715 | TGTCTGAGCAAAGGGAAGGCCAACG |
| TDR | 4,155,759 | 4,155,779 | CCTTCAACCCAACCCTGTAAC |
PCR Primers
| Primer | Sequence |
| SU | ATGCACACTTCCTCCCTTTG |
| SD | GAGAGGGCAGCTGTGAGAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 111 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13316A-A11 | VGB6 | C57BL/6NTac | |
| 13316A-B4 | VGB6 | C57BL/6NTac | |
| 13316A-C4 | VGB6 | C57BL/6NTac | |
| 13316A-D1 | VGB6 | C57BL/6NTac | |
| 13316A-F6 | VGB6 | C57BL/6NTac | |
| 13316A-F7 | VGB6 | C57BL/6NTac | |
