| Gene | Sele |
| Chromosome | 1 |
| Deletion Start | 164,048,689 |
| Deletion End | 164,055,787 |
| Deletion Size | 7,099 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 164,049,445 | 164,049,465 | CTGACAGAGGAAGCTCAGAAC |
| TUP | 164,049,466 | 164,049,487 | TGGGCTCCAGGTGAACCAAACA |
| TUR | 164,049,534 | 164,049,554 | TTCCACATGCCCGAGTCTTTG |
| TDF | 164,055,650 | 164,055,671 | TCACGTCCTTTCCTTGTGTTTC |
| TDP | 164,055,677 | 164,055,703 | CAGCTGCCAAAGCCTTCAATCGTTTGA |
| TDR | 164,055,707 | 164,055,729 | CGTTGTAAGAAGGCACATGGTAG |
PCR Primers
| Primer | Sequence |
| SU | AACGCCAGAACAACAATTCC |
| SD | GGAATAAAGGAAGAGGCCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13320A-B9 | VGB6 | C57BL/6NTac | |
| 13320A-C1 | VGB6 | C57BL/6NTac | |
| 13320A-C7 | VGB6 | C57BL/6NTac | |
| 13320A-E11 | VGB6 | C57BL/6NTac | |
| 13320A-E3 | VGB6 | C57BL/6NTac | |
