| Gene | Sell |
| Chromosome | 1 |
| Deletion Start | 164,062,192 |
| Deletion End | 164,079,709 |
| Deletion Size | 17,518 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 164,062,949 | 164,062,968 | GGGCTGATGGGCTTTATCTG |
| TUP | 164,062,974 | 164,062,995 | TGAAGAAGTGCCAGGGCACTGC |
| TUR | 164,063,064 | 164,063,086 | CCCAGTAAGTACCCTCACATCTC |
| TDF | 164,078,771 | 164,078,793 | GCAAAGGCACAGAAATATGGTTC |
| TDP | 164,078,795 | 164,078,818 | CCTCCACCCTTTCCAGGAATGGCA |
| TDR | 164,078,821 | 164,078,843 | GCCACTGTGTTCTATGTCACAAC |
PCR Primers
| Primer | Sequence |
| SU | AATCCAGTTGCCATTGCTTC |
| SD | TGGCATTAGCTTCTGTGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 138 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13321A-A5 | VGB6 | C57BL/6NTac | |
| 13321A-A9 | VGB6 | C57BL/6NTac | |
| 13321A-F6 | VGB6 | C57BL/6NTac | |
| 13321A-F2 | VGB6 | C57BL/6NTac | |
| 13321A-F1 | VGB6 | C57BL/6NTac | |
