| Gene | Tbxas1 |
| Chromosome | 6 |
| Deletion Start | 38,919,156 |
| Deletion End | 38,951,925 |
| Deletion Size | 32,770 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 38,919,226 | 38,919,246 | TGGCCCTCCTGAAATGGTAAG |
| TUP | 38,919,249 | 38,919,268 | TGGCCAACCACAGGGACTGC |
| TUR | 38,919,271 | 38,919,293 | CTCCTGGGCTGACATTTGTAGTG |
| TDF | 38,951,361 | 38,951,379 | TGGCCTCATGCACATCTTC |
| TDP | 38,951,380 | 38,951,405 | TGGCACCCTAGAGTTTCTGCCTATGA |
| TDR | 38,951,406 | 38,951,423 | GGCTCTCAGCAGGACTTC |
PCR Primers
| Primer | Sequence |
| SU | ACGGCGTATTTGAGAAGCAG |
| SD | AAGGGATGGAACATGACAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 63 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13324A-A3 | VGB6 | C57BL/6NTac | |
| 13324A-A7 | VGB6 | C57BL/6NTac | |
| 13324A-A9 | VGB6 | C57BL/6NTac | |
| 13324A-B7 | VGB6 | C57BL/6NTac | |
| 13324A-B9 | VGB6 | C57BL/6NTac | |
| 13324A-C10 | VGB6 | C57BL/6NTac | |
