| Gene | Adora1 |
| Chromosome | 1 |
| Deletion Start | 134,234,352 |
| Deletion End | 134,202,865 |
| Deletion Size | 31,488 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 134,234,251 | 134,234,272 | ACTGGTGATTTGGGCTGTGAAG |
| TUP | 134,234,227 | 134,234,249 | TGAACCAGGCACTTCGCGATGCT |
| TUR | 134,234,198 | 134,234,218 | ACCGCCAGGGATACGATGAAG |
| TDF | 134,203,448 | 134,203,465 | GGATAGCCAACGGCAGTG |
| TDP | 134,203,415 | 134,203,441 | AGCCCGTGATCAAGTGTGAGTTCGAGA |
| TDR | 134,203,367 | 134,203,391 | CCCAGACGAAGAAGTTGAAGTAGAC |
PCR Primers
| Primer | Sequence |
| SU | CAGTGGTGGAGTGAGTGCTG |
| SD | CCCTGTCTGACTGAAGGAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13326A-B9 | VGB6 | C57BL/6NTac | |
| 13326A-D6 | VGB6 | C57BL/6NTac | |
| 13326A-D11 | VGB6 | C57BL/6NTac | |
| 13326A-G1 | VGB6 | C57BL/6NTac | |
| 13326A-G7 | VGB6 | C57BL/6NTac | |
