| Gene | Siglec1 |
| Chromosome | 2 |
| Deletion Start | 131,086,095 |
| Deletion End | 131,069,799 |
| Deletion Size | 16,297 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 131,085,738 | 131,085,759 | CCTGCTCATTCCCTGCATCTTC |
| TUP | 131,085,711 | 131,085,735 | CTACCCTGCCGATGTCCCAGTGTCC |
| TUR | 131,085,667 | 131,085,687 | CGCTTGCCCGAGTAGTCATAG |
| TDF | 131,070,085 | 131,070,106 | CAGGTCTGAGCTTGTCTGATTC |
| TDP | 131,070,058 | 131,070,083 | TGTCACCTAGGCAGGAATGAAAGGCC |
| TDR | 131,070,026 | 131,070,048 | TGCAGGAGAGGATACATTAGGAG |
PCR Primers
| Primer | Sequence |
| SU | CCGACTTGTAGCTGGAGAGG |
| SD | AATCAAAGGCAACACTTGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13329A-E11 | VGB6 | C57BL/6NTac | |
| 13329A-H1 | VGB6 | C57BL/6NTac | |
| 13329A-F12 | VGB6 | C57BL/6NTac | |
| 13329A-F6 | VGB6 | C57BL/6NTac | |
| 13329A-H12 | VGB6 | C57BL/6NTac | |
| 13329A-G2 | VGB6 | C57BL/6NTac | |
