| Gene | Lama1 |
| Chromosome | 17 |
| Deletion Start | 67,697,338 |
| Deletion End | 67,717,269 |
| Deletion Size | 19,932 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 67,697,781 | 67,697,801 | GCCGGGTTGGTTGATTGTATG |
| TUP | 67,697,803 | 67,697,827 | AGCGCTCCCGCAATGAATGGGAACG |
| TUR | 67,697,830 | 67,697,852 | CAATGAAGTGAATGCCAGAGAAC |
| TDF | 67,716,810 | 67,716,829 | CCTGTTCGACACGCCCAATG |
| TDP | 67,716,831 | 67,716,855 | CGGGTCTGTGACGGTAACAGTACGA |
| TDR | 67,716,914 | 67,716,933 | GCACCCGCAAAGAGAAGTTG |
PCR Primers
| Primer | Sequence |
| SU | AGGGTCAGAGTACCACCCAG |
| SD | CCGTATAGGTGAAGAAGTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 124 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13332A-G4 | VGB6 | C57BL/6NTac | |
| 13332A-C6 | VGB6 | C57BL/6NTac | |
| 13332A-B6 | VGB6 | C57BL/6NTac | |
| 13332A-E6 | VGB6 | C57BL/6NTac | |
