| Gene | Epha10 |
| Chromosome | 4 |
| Deletion Start | 124,883,906 |
| Deletion End | 124,915,744 |
| Deletion Size | 31,839 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 124,884,515 | 124,884,533 | CAGGAGAACTGGCTGTGTC |
| TUP | 124,884,535 | 124,884,560 | ACGTACAGAAATGTGAGCTCCGTGGA |
| TUR | 124,884,562 | 124,884,580 | AGGCAGTGGGCAGCATTTG |
| TDF | 124,914,963 | 124,914,982 | CCACCTCCTCGTACCTGTTC |
| TDP | 124,914,983 | 124,915,008 | AGGTGTGAAGCTCAGCCCATTGTCCA |
| TDR | 124,915,013 | 124,915,034 | CCTGAAGCAGGGATGTCAGATG |
PCR Primers
| Primer | Sequence |
| SU | TCACAAGCCTCAGGGAATGAA |
| SD | AGGACAACAACTGAAACTCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 66 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13336A-F3 | VGB6 | C57BL/6NTac | |
| 13336A-H11 | VGB6 | C57BL/6NTac | |
