| Gene | Ephb6 |
| Chromosome | 6 |
| Deletion Start | 41,613,319 |
| Deletion End | 41,620,391 |
| Deletion Size | 7,073 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 41,614,023 | 41,614,042 | TGTCACCTCCCTCTGCAAAC |
| TUP | 41,614,043 | 41,614,071 | TCTGAGCAGTTATTGTTTCTCCTGGTCCC |
| TUR | 41,614,076 | 41,614,097 | GAACACTCACCTCATCCCACTG |
| TDF | 41,620,245 | 41,620,265 | CTGCACAACATTCAGCTCCTC |
| TDP | 41,620,266 | 41,620,287 | CAGCAGCACCTGAGGCAGCCAG |
| TDR | 41,620,316 | 41,620,334 | ATCGGCGGCTCACAGACTC |
PCR Primers
| Primer | Sequence |
| SU | AGATGAATCAACCCTTACTGG |
| SD | TAAGTGGGAGGGCACAGAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13337B-B7 | VGB6 | C57BL/6NTac | |
| 13337B-F7 | VGB6 | C57BL/6NTac | |
| 13337B-D7 | VGB6 | C57BL/6NTac | |
| 13337B-C3 | VGB6 | C57BL/6NTac | |
| 13337B-F1 | VGB6 | C57BL/6NTac | |
