13341   Tmprss2     Status: Germline transmission achieved

GeneTmprss2
Chromosome16
Deletion Start97,599,266
Deletion End97,566,135
Deletion Size33,132
Genome Build38
MGI IDMGI:1354381
NCBI Gene ID0
BAC ID91C4
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 16

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF97,598,61397,598,635TCTGGATGAGGTATTGGGTAGTC
TUP97,598,58397,598,609TCCTGCTGGAACTCTTCTCCTTAGGGC
TUR97,598,54897,598,569CCGGCTTTAAGGTGACAGAGAC
TDF97,566,52297,566,542GGTTGCACAGCAAGGAAGAAG
TDP97,566,49797,566,520CCAGGAGTTCCTGTGAGCCTACCC
TDR97,566,44897,566,467TGGAATGGAAGGAGCTGGAG

PCR Primers

PrimerSequence
SUAGGTGCTCCTCTGTAGGCAG
SDGATTTAGGCCACAGGCAATG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR88 bp++-
TDFTDR95 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
13341C-H6VGB6C57BL/6NTac
13341C-B6VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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