| Gene | Aqp11 |
| Chromosome | 7 |
| Deletion Start | 97,737,984 |
| Deletion End | 97,726,541 |
| Deletion Size | 11,444 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 97,737,572 | 97,737,592 | GTACTGCATAAGCGCCCTGTG |
| TUP | 97,737,539 | 97,737,566 | TGAGTCTGACCAAGTACCATTACGACGA |
| TUR | 97,737,499 | 97,737,520 | CATGTCGGTGTGGATGGGATTC |
| TDF | 97,726,601 | 97,726,624 | CCACTCCCAAAGACTCGAACTAAG |
| TDP | 97,726,577 | 97,726,600 | TCCCAGGACAGTCAAGCTGGATGC |
| TDR | 97,726,557 | 97,726,576 | TGGAGGGTGCTCAGATTGTC |
PCR Primers
| Primer | Sequence |
| SU | ACATGCTCTAGCACCAGCG |
| SD | AAGAAGCTGTGGCTGGAAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 13346B-F3 | VGB6 | C57BL/6NTac | |
| 13346B-G3 | VGB6 | C57BL/6NTac | |
